Description:
The vector pKLMF-EK directs high-level intracellular expression of a recombinant protein fused to the maltose binding protein (MBP) in the yeast Kluyveromyces lactis. It is compatible with the K. lactis Protein Expression Kit (NEB #E1000). MBP fusion proteins expressed from pKLMF-EK can be affinity purified from cell lysates using Amylose Resin (NEB #E8021). MBP can be removed from purified fusion proteins by digestion with Enterokinase (NEB #P8070).