• PLAC4-PBI promoter does not express in E. coli allowing toxic genes to be cloned prior to their expression in yeast.
• Universal MCS lies downstream of α-MF secretion domain and PLAC4-PBI promoter.
• Acetamidase expression for non-antibiotic selection in K. lactis.
• Ampicillin resistance for propagation in E. coli. (See notes.)
Description:
The vector pKLAC2 directs high-level expression of a recombinant protein from the yeastKluyveromyces lactis and is compatible with the K. lactis Protein Expression Kit (NEB #E1000). pKLAC2 can be used for either intracellular or secreted protein expression. SacII or BstXI linearized pKLAC2 integrates into the LAC4 locus of the K. lactis genome upon transformation of K. lactis competent cells. pKLAC2 differs from pKLAC1 (NEB #N3740) in that it contains a universal multiple cloning site (MCS) that is compatible with all NEB expression systems.
Source:
pKLAC2 is isolated from E. coli strain ER2268 by a standard DNA purification procedure.
Concentration:
1,000 μg/ml
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C
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