Description:
The vector pKLAC1 directs high-level expression of a recombinant protein from the yeast Kluyveromyces lactis and is part of the K. lactis Protein Expression Kit (NEB #E1000). pKLAC1 can be used for either intracellular or secreted protein expression. SacII or BstXI linearized pKLAC1integrates into the LAC4 locus of the K. lactis genome upon transformation of K. lactis GG799 Competent Cells (NEB #C1001).
Source:
pKLAC1 is isolated from E. coli strain ER2268 by a standard DNA purification procedure.
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C