Description:
MidRange PFG Marker I is concatemers of λ DNA isolated from the bacteriophage λ (cI857 ind1 Sam7) mixed with XhoI digested λ DNA embedded in 1% LMP agarose and supplied in a GelSyringe™ dispenser. XhoI produces fragments of 15.0 and 33.5 kb. These fragments anneal to and form concatemers with intact λ DNA. It is designed for use as a size marker for pulsed field gel electrophoresis (PFG). Size range: 15-300 kb.
Effective Size Range: 1 kb to 291 kb
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
50% Glycerol
1% LMP agarose
pH 8.0 @ 25°C