LITMUS 38i is a multi-purpose cloning/in vitrotranscription phagemid vector. The molecule is a small, double-stranded circle 2,814 base pairs in length (molecular weight =1.8 x 106daltons).
Highlights
Extensive set of restriction sites in polylinker, many with unique 4-base overhangs
Blue/white selection
Opposing T7 promoters for making RNA transcripts in either direction or double-stranded RNA using the HiScribe RNAi Transcription Kit (NEB #E2000S)
Product Source
LITMUS 38i is isolated fromE. coliER2738 by a standard plasmid purification procedure.
Features
Small (< 3 kb), high copy number
Ampicillin resistance
Single-stranded (m13) DNA replication origin
Compatible with pUC/M13 sequencing primers as well as LITMUS sequencing primers