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LITMUS 38i Vector

 
包装: 20 μg
运保温度: -20°C
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描述:

LITMUS 38i is a multi-purpose cloning/in vitro transcription phagemid vector. The molecule is a small, double-stranded circle 2,814 base pairs in length (molecular weight =1.8 x 106 daltons).
 

Highlights

  • Extensive set of restriction sites in polylinker, many with unique 4-base overhangs 
  • Blue/white selection
  • Opposing T7 promoters for making RNA transcripts in either direction or double-stranded RNA using the HiScribe RNAi Transcription Kit (NEB #E2000S)

Product Source

LITMUS 38i is isolated from E. coli ER2738 by a standard plasmid purification procedure.

Features

  • Small (< 3 kb), high copy number
  • Ampicillin resistance
  • Single-stranded (m13) DNA replication origin 
  • Compatible with pUC/M13 sequencing primers as well as LITMUS sequencing primers

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C


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