Description:
TriDye™ 100 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
The DNA ladder consists of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100-1,517 base pairs. The 500 and 1,000 base pair bands have increased intensity to serve as reference points.
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.
Effective Size Range: 100 bp to 1,517 bp
Source:
The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Concentration:
50 μg/ml
Recommended Load: 10 μl
Storage Conditions:
0.006% Xylene Cyanol FF
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% Bromophenol Blue
0.06% Orange G
pH 7.9 @ 25°C
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