Description:
TriDye™ 2-Log DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis.
The DNA Ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 100 bp to 10 kb. The 0.5, 1.0 and 3.0 kb bands have increased intensity to serve as reference points.
TriDye During Electrophoresis: On a standard 1% agarose gel in 1X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp and the orange G at approximately 50 bp. As the percentage of agarose changes, the migration rates of the dyes relative to migration rates of the DNA will change.
Effective Size Range: 100 bp to 10,002 bp
Source:
The TriDye 2-Log DNA Ladder was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
Concentration:
100 μg/ml
Recommended Load: 10 μl
Storage Conditions:
0.006% Xylene Cyanol FF
10 mM Tris-HCl
10 mM EDTA
10% Glycerol
0.006% Bromophenol Blue
0.06% Orange G
pH 7.9 @ 25°C
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