Description:
pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
GenBank Assession Number: L09137
Source:
pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M-) by a standard plasmid purification procedure.
General Utility: Cloning
Selectable Markers: Ampicillin
Origin of Replication: ColE1
Copy Number: High (100+)
Molecular Weight:
Theoretical: 1,750,000 daltons
Concentration:
1,000 μg/ml
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C