Description:
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight is 2.83 x 106 daltons.
*Sequencing data from Watson (confirmed at New England Biolabs, Inc.) has shown pBR322 to be 4,361 base pairs, not 4,363 base pairs as previously reported.
Source:
pBR322 is isolated from E. coli ER2272 (dam +dcm + EcoK M-) by a standard plasmid purification procedure.
Concentration:
1,000 μg/ml
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
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