Description:
The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI. The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. These fragments are best separated by Pulsed Field Gel Electrophoresis.The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Effective Size Range: 1,503 bp to 48,502 bp
Source:
The phage is isolated from the heat- inducible lysogen E. coli λ c1857 S7 and then isolated from the purified phage by phenol extraction and dialyzed. The double-stranded DNA is then digested to completion with the appropriate enzyme, phenol extracted and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Reagents Supplied:
Gel Loading Dye, Blue (6X)
Concentration:
500 μg/ml
Recommended Load: 0.5 μg
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
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