Description:
The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.
Source:
The phage is isolated from the heat-inducible lysogen E. coli λ cI857 S7 and then isolated from the purified phage by phenol extraction and dialyzed. The double-stranded DNA is digested to completion with HindIII, phenol extracted and dialyzed against 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Effective Size Range: 125 bp to 23,130 bp
Reagents Supplied:
Gel Loading Dye, Blue (6X)
Concentration:
500 μg/ml
Recommended Load: 1 μg
Storage Conditions:
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C
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