Recombinant RNasin? Ribonuclease Inhibitor

产品编号：N2511 品牌：promega 原厂货号：N2511
产品分类：克隆 > DNA/RNA修饰酶 > RNA酶抑制剂
应用分类：PCR > 反转录 > RNase 抑制剂

包装：

2,500u

运保温度:

–20°C

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其它组分：

Recombinant RNasin® Ribonuclease Inhibitor【子货号：N251A，包装：1 x 2,500u，，运保温度：–20°C】

描述：

说明

天然的和重组的RNasin^® 核酸酶抑制剂具有广谱的抑制RNase 活性的性质，包括抑制中性型(neutral type) 真核RNase。该抑制剂的分子量为50kDa，按1:1 的比例与RNase 非共价结合发挥抑制作用。RNasin^® Ribonuclease Inhibitor 与RNase( 如RNase A) 结合的Ki 值大约是10-14M。Promega 提供两种不同的抑制剂：天然的RNasin^® Ribonuclease Inhibitor 和重组的RNasin^® Ribonuclease Inhibitor。两种产品均通过离子交换和亲和层析相结合的纯化方法纯化得到。

特点

• 抑制常见的真核RNase：RNasin^® Ribonuclease Inhibitor 具有广谱RNase 抑制特性，包括 RNase A、 RNase B、 RNase C 和人类胎盘RNase，不抑制RNase T1、 S1 核酸酶、曲霉(Aspergillus ) 中的 RNase 以及RNase H、RNase ONE^®Ribonuclease。

• 兼容性：RNasin^® Ribonuclease Inhibitor 不抑制SP6、T7 或T3RNA Polymerase、ImProm-II™、AMV 或M-MLV Reverse Transcriptase 或Taq DNA Polymerase。

• pH 范围宽：在较宽的pH 范围内(pH 5-8) 均可发挥作用。

原厂资料：

RNases are ubiquitous, cause RNA degradation and can compromise RNA integrity. Recombinant RNasin® Inhibitor is a 50kDa protein that inhibits RNase A family and human placental RNases by noncovalently binding to RNases in a 1:1 ratio. Recombinant RNasin® Inhibitor does not inhibit RNase T1, S1 nuclease, RNase from Aspergillus, RNase H, RNase ONE™ Ribonuclease and enzymes for downstream applications such as GoScript™ Reverse Transcriptase, AMV/M-MLV reverse transcriptases, SP6, T7/T3 RNA polymerase, and Taq DNA polymerases. Learn more about our custom options for this product at: www.promega.com/custom/

Features - Benefits

Broad-Spectrum RNase Inhibition: Inhibits common eukaryotic RNases.
Compatible: Does not inhibit SP6, T7 or T3 RNA Polymerase; GoScript™ Reverse Transcriptase, AMV or M-MLV Reverse Transcriptase; or Taq DNA polymerase.
Flexible: Functional across wide pH range (pH 5–8) enabling use in many downstream assays.
Recombinant: Made in bacteria, minimizing the chances of human nucleic acid contamination.
Applications
RT-PCR and RT-qPCR.

Useful in any applications where eukaryotic RNase contamination is a potential problem.
Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation.
Increase yield and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Performing RNase-free antibody staining.
Enhancement of RNA yields from Riboprobe® System RNA synthesis reactions.

References

Blackburn, P. and Moore, S. (1982) In: The Enzymes, Vol. XV, Part B.
Blackburn, P. et al. (1977) J. Biol. Chem. 252, 5904–10.
Lee, F.S. et al. (1989) Biochemistry 28, 219–24.
Lee, F.S. et al. (1989) Biochemistry 28, 225–30.

注意事项：

QC Tests
SDS-PAGE/purity, DNase, RNase, latent RNase, endonuclease.

Source
Recombinant strain of E. coli (Cat.# N2511, N2515).

Storage Buffer
20mM HEPES-KOH (pH 7.6 at 4°C), 50mM KCl, 8mM DTT and 50% (v/v) glycerol.

Storage Conditions
Store at –20°C.

Unit Definition
One unit is defined as the amount of RNasin® Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2,3´-cyclic monophosphate by ribonuclease A.

For product intended use please see Patents & Disclaimers tab.