pNL3.2.CMV[NlucP/CMV] Vector

产品编号：N1411 品牌：promega 原厂货号：N1411
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
应用分类：

包装：

20ug

运保温度:

–20°C

到货周期：

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其它组分：

pNL3.2.CMV Vector【子货号：N141A，包装：1 x 20μg，，运保温度：–20°C】

描述：

The pNL3.2.CMV Vector(a,b) is designed for constitutive expression of the NanoLuc® reporter fused to a PEST destabilization domain (NlucP). The NlucP fusion protein naturally accumulates at low intracellular levels due to constitutive proteosomal degradation, thus serving as a negative control for experiments configured to measure regulated changes in NanoLuc® luciferase expression levels. This vector also encodes a hygromycin resistance gene for selection in mammalian cells and an ampicillin resistance gene for selection in bacteria. The pNL3.2.CMV Vector contains the following features:
• A CMV promoterfor robust expression in mammalian cells
• The NanoLuc® reporter gene, for high sensitivity detection of the protein of interest.
• PEST sequence for maintaining low intracellular NanoLuc® luciferase concentration.
• Ampicillin resistance gene for selection in bacteria.
• Hygromycin resistance gene for selection in mammalian cells.