Acyclonucleotide Set

产品编号：N0460S 品牌：New England Biolabs 原厂货号：N0460S
产品分类：PCR/RT-PCR/qRT-PCR > 其它 > 核苷酸 > dNTPs
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描述：

Description:
Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5). When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ .