Description:
Deoxynucleotide Solution Mix is an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP.
Supplied In: Ultrapure water as a sodium salt at pH 7.5.
Reagents Supplied:
10 mM Deoxynucleotide Solution Mix 5 x 0.8 ml (large); 4 x 0.2 ml (small)
Example of Use: For a reaction which calls for 200 µM of each dNTP, add 2 µl of this premixed nucleotide solution per 100 µl reaction.
Diluent Compatibility: Can be diluted using sterile distilled water, preferably Milli-Q™ water or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5).
Concentration:
10 mM each nt
Storage Conditions:
10 mM Milli-Q water
pH 7.5 @ 25°C