pCLuc-MiniTK 2 Vector

• 产品编号：N0319S      品牌：New England Biolabs       原厂货号：N0319S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 哺乳动物表达载体
• 应用分类：

描述：

Description: 
pCLuc Mini-TK 2 is a cloning vector for mammalian cells, containing a minimal promoter fragment from the HSV thymidine kinase (TK) promoter adjacent to a reporter gene, the secreted luciferase from the Ostracod Cypridina noctiluca.Cypridina luciferase (CLuc) is a 62 kDa protein with a native signal peptide at the N-terminus that allows it to be secreted from mammalian cells (1) so that CLuc activity can be detected in the culture medium of mammalian cells expressing the reporter gene. The pCLuc Mini-TK 2 Vector contains a MCS upstream of the minimal TK promoter for cloning promoter or enhancer elements. A neomycin resistance gene under the control of an SV40 promoter allows selection for stable integration of the plasmid into the mammalian cell genome using G418.

Recommended sequencing primers for pCLuc Mini-TK 2 Vector (not available from NEB) 

Upstream of MCS: 
pGLuc Basic Forward Sequencing Primer (23-mer)
GGGGTTCCGCGCACATTTCCCCG (6090–6112) 

pBasic Reverse Primer (25-mer)
TCAGAAGCCATAGAGCCCACCGCAT (1958–1934) 

CLuc 3´ end Forward Primer (23-mer)
GAGTTCAAGAAAGAATGCTACAT (1741–1763) 

CLuc 5´ End Reverse Primer (24-mer)
GTAAGGACAGTCCTGGCAATGAAC (213–190)

原厂资料：