Figure . Silencing potency of siRNAs containing ON-TARGET™ modifications. (A) Three different siRNAs, targeting human
cyclophilin B mRNA, were individually tested in a) unmodified, b) antisense strand modi-fied, and c) sense strand modified
forms for their ability to direct mRNA degradation in HEK293 cells. In all three cases, unmodified duplexes efficiently silenced the target. When ON-TARGET™ Modifications were applied to the antisense strand (S-*AS), duplex functionality was
eliminated, probably because the antisense strand (the strand responsible for directing gene-specific silencing) is unable to associate with the RISC. In contrast, when the same modifications are applied to the sense strand only (*S-AS), the silencing potency of the duplex is maintained or increased (ns = nonspecific; Control = non-targeting siRNA).(B) A heat map resulting from microarray studies illustrates the off-target expression profile of cells treated with unmodified and modified siRNAs directed against Gene A. Note that in this case, modification of the sense strand eliminates the majority of the off-target signature (illustrated primarily in the boxed region). When only the antisense strand is modified, off-target effects occur (blue = down-regulation, black = no effect, pink = up-regulation). (Heat map data presented with permission from A. Jackson and P.S. Linsley, Rosetta Inpharmatics LLC.) B.
Thermo Scientific siGENOME siRNA reagents are widely recognized and trusted for highly efficient target gene silencing. Our scientists were the first to establish siRNA design rules for high potency silencing and continue to drive innovation in this area. By providing genome-wide siRNAs designed with the SMARTselection algorithm, researchers can focus on successful RNAi experiments instead of optimizing siRNA designs for their genes of interest.
siGENOME siRNA is available as pre-designed, genome-wide SMARTpool and individual siRNA reagents for human, mouse and rat. Simply search for your gene of interest and choose from the available product formats and quantities.
Highlights
• Guaranteed knockdown by SMARTpool and 3 of 4 individual siRNAs (see Specifications)
• Antisense strand loading into RISC ensured by thermodynamic analysis and selective application of a sense strand-blocking modification (ON-TARGET)
• Sequence information provided with siRNA purchase
Design and modification strategies for optimal silencing
In addition to identifying potent siRNA designs with the SMARTselection algorithm, the creation of siGENOME siRNAs also involves:
• Thermodynamic analysis to determine optimal antisense strand-loading characteristics for effective silencing
• BLAST analysis of both strands to alleviate cross-targeting
To retain more high-scoring siRNA designs, the above analysis may trigger the use of a sense strand-blocking modification (ON-TARGET) to ensure only the appropriate strand enters RISC for effective target knockdown (see Figure 2, Supporting Data).
Available Product Formats
•SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus guaranteed to silence by 75% or better (see Specifications).
•Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed to silence by 75% or better (see Specifications).
•Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.
•Individual siRNAs: Select 1, 2, 3 or 4 individual siRNAs per gene. Minimum purchase of 4 at the 2 nmol size.
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