MCA1129 reacts strongly to purified secretory - piece and secretory IgA. No reaction against myeloma IgA (native or reduced and alkylated). Negative against normal human IgM, myeloma IgM, normal human IgG; free kappa or gamma chains, and J-chain. Typical pattern on frozen and paraffin embedded sections of intestinal and breast tissue.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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