MCA1123PE is suitable for use as a negative control reagent to assess the non-specific binding of rat IgG1 to human tissues. Not suitable for use as a negative control to assess binding of rat IgG1 to mouse tissues.
Test results have shown that MCA1123PE is also suitable as a negative control with porcine and canine tissues.
Fusion Partners
Spleen cells from an immunised Lou/c rat were fused with cells of the rat IR983F myeloma cell line.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells or 100ul whole blood