MCA1118C recognises CD86, a 70kD cell surface protein expressed by activated B cells and monocytes. CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in co-stimulation of T cells in the primary immune response.
Fusion Partners
Spleen cells from immunised mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5ul of the suggested working dilution to label 106cells in 100ul.
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