MCA1109 reacts with human IL-8, also known as CXCL8. Clone B-K8 is reported to block the chemotaxis of neutrophils. Removal of Sodium Azide is recommended prior to use in functional assays. Serotec recommend the use ofEQU003for this purpose.
Fusion Partners
Spleen cells from immunised BALB/c (Iffa Credo) mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen(1)
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. AbD Serotec recommends the use of acetone fixation for frozen sections.