MCA1054GA recognises CD59, a PI-linked glycoprotein, Mw 18-20kD, which is found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. Clone MEM-43 has been shown to stimulate NK activity.
* The epitope recognised by MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2, 5).
MEM-43 has been reported to be suitable for use in IHC on paraffin sections.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
1/5 - 1/10
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting(1)
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
(1)
This product recognizes CD59 under non-reducing conditions.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.