MCA1043A488, clone YKIX 753.22.2, was clustered as canine CD45R in the First Canine Leucocyte Antigen Workshop (CLAW). It immunoprecipitates an antigen of 200kD from Con-A blast, seeCobbold, S. & Metcalfe, S.for details. CD45 is expressed on all leukocytes in canine peripheral blood and may be against the CD45R isoforms.CD45R is expressed on subsets of leukocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.
Fusion Partners
Spleen cells from immunised DA rat were fused with cells of the Y3/A1.2.3 rat myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Neat - 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
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