RAT ANTI DOG CD4:FITC

产品编号：MCA1038F 品牌：AbD Serotec 原厂货号：MCA1038F
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

100 TESTS

运保温度:

+4oC or -20oC

到货周期：

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描述：

Specificity

Rat anti Dog CD4 antibody (YKIX302.9) is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4.

Rat anti Dog CD4 (YKIX302.9) has been demonstrated to partially deplete circulating T lymphocytes when administered in vivo, but alone was not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993).

Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocytes subsets Mooreet al. 1992. Rat anti Canine CD4 (YKIX302.9) forms part of a panel of anti canine monoclonal antibodies used extensively in the evaluation of leukemic status in dogs (Villiers 2002).

Fusion Partners

Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Storage

Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
Flow Cytometry				Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul whole blood.