RAT ANTI DOG CD5:FITC

产品编号：MCA1037F 品牌：AbD Serotec 原厂货号：MCA1037F
产品分类：抗体 > 一抗 > 染料标记抗体
应用分类：

包装：

0.1 mg

运保温度:

+4oC or -20oC

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描述：

Specificity

Rat anti Dog CD5 antibody, clone YKIX322.3 recognises canine CD5, a 67kD cell surface type 1 transmembrane glycoprotein also known as Lymphocyte antigen T1, Ly-1 or Leu-1. CD5 is expressed on the surface of T cells and thymocytes, CD5 is also expressed by NK cells at low levels (Huang et al. 2008). Rat anti dog CD5, cloneYKIX322.3 was clustered as canine CD5 in the First Canine Leucocyte Antigen Workshop (Cobbold et al.1994).

In a study of 73 cases of canine chronic lymphocytic leukemia (CLL) CD5 expression was absent on all cases of B cell CLL as defined by CD21 expression and lack of CD3 or other T cell antigen expression (Vernau and Moore 1999). Rat anti dog CD5 serves as a useful marker for the discrimination of canine leukemias of differing origins (Villiers 2002).

Fusion Partners

Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.

Storage

Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life

18 months from date of despatch.

Application

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.

Application Name	Yes	No	Not Determined	Suggested Dilution
Flow Cytometry				Neat - 1/10

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10ul of the suggested working dilution to label 10⁶ cells or 100ul of whole blood.