MCA1029G recognises the OX-62 antigen, shown to be rat alpha E2 integrin which is expressed by dendritic cells of dendritic epidermal T cells, intraepithelial lymphocytes in the small intestine and by cells of denritic morphology in lymphoid organs, at sites where gamma delta T cells are present.
Fusion Partners
Spleen cells of BALB/c mice were fused with cells of the mouse NSO myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
1/50 - 1/100
Immunohistology - Frozen
1/25 - 1/100
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.
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