MCA07 recognises the RT6.1 alloantigen also known as ART2, Pta.A2, and Ag-F1. RT6 is expressed on most peripheral T cells but not on thymocytes. Clone P4/16 recognises rat strains expressing RT6.1 including BUF, LEW, and PVG and will not recognise RT6.2 expressing strains such as AUG, BDIX, DA and F344.
Fusion Partners
Spleen cells from an immunised AUG rat were fused with cells of the mouse P3/NS1/1-Ag4.1 myeloma cell line.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Neat - 1/10
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106cells in 100ul.