MCA04GT recognises mouse and rat CD90 also known as Thy1.1. Thy1.1 is a monomorphic determinant in rat but polymorphic in mice. Clone T11D7e reacts with Thy1.1 mice e.g. AKR and FVB mice, but not Thy1.2 mice such as CBA and BALB/c.
Clone T11D7e is useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity, seeLake, P.et al.for details.
This product is routinely tested in flow cytometry using mouse thymocytes.
Species Cross-Reactivity
Target Species
Cross Reactivity
Mouse
Yes
Fusion Partners
Spleen cells from immunised CBA mice were fused with cells of the mouse NS1 myeloma cell line.
Storage
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Cytotoxic Assays
ELISA
Flow Cytometry
1/5
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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