S1 Nuclease

• 产品编号：M5761      品牌：promega       原厂货号：M5761
• 产品分类：克隆 > DNA/RNA修饰酶 > 核酸酶
• 应用分类：

其它组分：

描述：

原厂资料：

S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme. The enzyme is used to remove single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts.

Features - Benefits

• Provided with 10X Reaction Buffer: 0.5M sodium acetate (pH 4.5 at 25°C), 2.8M NaCl, 45mM ZnSO₄.

Applications

• Removal of protruding single-stranded termini in double-stranded DNA.
• Selective cleavage of single-stranded DNA.
• Mapping RNA transcripts.

References

1. Vogt, V.M. (1973) Eur. J. Biochem. 33, 192–200.
2. Roberts, T.M. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 760–4.
3. Berk, A.J. and Sharp, P.A. (1978) Proc. Natl. Acad. Sci. USA 75, 1274–8.