GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when the amplification reactions are heated at 94–95°C for two minutes. This enables hot-start PCR, where polymerase activity is eliminated or minimized at temperatures below 70°C. GoTaq® Hot Start Polymerase exhibits 5´→3´ exonuclease activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so that samples sink easily into wells of an agarose gel. The green buffer also contains two dyes (yellow and blue) that separate to allow easy monitoring during electrophoresis. Use the green reaction buffer for direct-to-gel analysis after amplification and the colorless reaction buffer for amplifications where the dyes may interfere with post-amplification analysis such as fluorescence or absorbance testing.
GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Master Mixes offer the specificity and sensitivity of an antibody-based hot-start polymerase in a convenient, easy-to-use, time-saving format.
Features - Benefits
Enhanced Yield, Sensitivity and Specificity: The proven, robust amplification and sensitivity of GoTaq® DNA Polymerase now with built-in hot start to deliver even more superior results.
Ease of Use: Set up your reaction at room temperature—no need to set up on ice.
Higher Yield: Two-minute activation saves time and ensures maximum enzyme activity.
Higher Specificity: Minimize nonspecific amplification and primer-dimers.
Improve Productivity: Go directly from PCR to gel analysis. Green GoTaq® Reaction Buffer serves as both reaction buffer and gel-loading solution.
Convenience: One tube, one pipetting step. Only add template and primers when using the master mixes.
Optimization: Control the magnesium concentration in your reaction for specialized templates when using the standalone polymerase.
Guarantee
PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account. That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests preformed.
Applications
Hot-start PCR.
Complex genomic templates.
T-vector cloning.
RT-PCR.
Two-step RT-PCR.
Low-copy target.
GoTaq® Hot Start Colorless Master Mix is a premixed ready-to-use solution containing GoTaq® Hot Start Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets, because it may eliminate or minimize primer-dimer and secondary products. In some cases, hot-start PCR may improve yields.
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