Ribonuclease H

• 产品编号：M4281      品牌：promega       原厂货号：M4281
• 产品分类：克隆 > DNA/RNA修饰酶 > RNase
• 应用分类：

其它组分：

描述：

Ribonuclease H (RNase H，核糖核酸酶H）是一种核酸内切酶，特异性地水解与DNA 杂交的RNA 的磷酸二酯键，产生 3`-OH 和5`-P末端的产物。它不能降解单链核酸、双链DNA 或双链RNA。

原厂资料：

Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA.

Applications

• Removal of the RNA strand prior to second-strand cDNA synthesis.
• Analysis of in vitro polyadenylation reaction products.
• Microarrays.

References

1. Goodwin, E.C. and Rottman, F.M. (1992) Nucl. Acids Res. 20, 916.