GoTaq? Long PCR Master Mix

产品编号：M4021 品牌：promega 原厂货号：M4021
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 长片段PCR
应用分类：PCR > PCR酶和预混液 > PCR预混液
　　　　　 PCR > PCR酶和预混液 > PCR预混液 > 长片段PCR预混液

包装：

100 reactions

运保温度:

–20°C

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1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

其它组分：

Nuclease-Free Water【子货号：P119E，包装：1 x 13ml，，运保温度：–20°C】

Human Genomic DNA【子货号：G304B，包装：1 x 30μl，，运保温度：–20°C】

GoTaq® Long PCR Master Mix, 2X【子货号：M402A，包装：2 x 1.25ml，，运保温度：–20°C】

Long PCR Control Primer Pair【子货号：M412A，包装：1 x 50μl，，运保温度：–20°C】

描述：

说明

GoTaq^® Long PCR Master Mix （GoTaq^® 长片段 PCR Master Mix ）包含高性能的GoTaq^® 热启动聚合酶，与专利的耐热且具有校正功能的聚合酶一起，形成特殊配方的混合物。这个优化的酶混合物既可以有效扩增长达40kb 的λDNA ，也可以有效扩增30kb 的人类基因组DNA 。在快速扩增的聚合酶存在时，具有校正功能的酶可以修复DNA 错配，使聚合酶在DNA 上持续延伸得更远，从而得到更长的DNA 扩增片段。

GoTaq^®长片段PCR Master Mix 组分的优化配方经过改造，可以进行单个反应的组装，从而提供稳定、有效、准确、强劲的长片段DNA 扩增性能。

特点

备受肯定的GoTaq^® 聚合酶的强劲扩增性能现在可以用于长片段PCR 的扩增（长达30kb 的gDNA ）。

• 方便：热启动Master mix 操作方便，可以单独组装。

• 性能提高：使用优化的组分，产量、灵敏度和特异性得到提高。

• 准确：将耐热的DNA 聚合酶与增强的延伸功能与校正功能结合起来。

• 可靠：提供对照引物对和人类gDNA 模板，控制反应并能检测模板的质量。

• 高效：是基因克隆、突变分析以及DNA 测序的最佳选择。

操作手册

GoTaq^® Long PCR Master Mix Technical Manual

储存条件

收到产品后，将所有组分避光储存于 –20°C 。若立即使用，可将组分避光储存于 2–8°C ，最长可达 3 个月。

专利/免责声明

Limited Label License: The purchase of this product conveys to the purchaser only the limited, non-transferable right to use the purchased quantity of the product for the purchaser`s own research by the purchaser only under the following U.S. patent claims and foreign counterpart patent claims: U.S. Patent No. 5,436,149 (claims 6-16). No rights are granted to the purchaser to sell, modify for resale or otherwise transfer this product, either alone or as a component of another product, to any third party. Takara Bio reserves all other rights, and this product may not be used in any manner other than as provided herein. For information on obtaining a license to use this product for purposes other than research, please contact Takara Bio Inc., Seta 3-4-1, Otsu, Shiga 520-2193, Japan (Fax +81-77-543-9254).

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

Licensed under U.S. Pat. Nos. 5,338,671 and 5,587,287 and corresponding patents in other countries. For Research Use Only. Not for use in diagnostic procedures.

Licensed under U.S. Pat. Nos. 5,556,772, 7,297,519 and 7,312,052 for in vitro research use only.

相关产品

• GoTaq^® Hot Start Polymerase

• GoTaq^® Amplification Family

• Wizard^® SV 96 and SV 9600 Plasmid DNA Purification Systems

原厂资料：

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermostable proofreading polymerase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polymerase allows the polymerase to continue to elongate the DNA much further, resulting in longer DNA amplification.

The optimized formulation of the GoTaq® Long PCR Master Mix components enables simple reaction setup and provides consistently efficient, accurate and robust amplification of long DNA amplicons.

Features - Benefits

The proven robust amplification using GoTaq® Polymerase is now available for long-range PCR (up to 30kb gDNA).

Easy: Hot-start master mix for convenient handling and simple setup.
Enhanced: Yield, sensitivity and specificity with optimized components.
Accurate: Blend of thermostable DNA polymerases with enhanced processivity and proofreading.
Confident: Control primer pair and human gDNA template to perform control reactions and test template quality.
Efficient: Perfect for cloning genes, mutational analysis and DNA sequencing.

注意事项：

储存条件

收到产品后，将所有组分避光储存于 –20°C 。若立即使用，可将组分避光储存于 2–8°C ，最长可达 3 个月。