Single-Stranded DNA Binding Protein

• 产品编号：M3011      品牌：promega       原厂货号：M3011
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 其它纯化的蛋白及酶
• 应用分类：

其它组分：

描述：

E. coli Single-Stranded DNA Binding Protein(SSB，大肠杆菌单链DNA 结合蛋白) 由4 个相同的18.9kDa 的亚基组成。它以协同方式与单链DNA 高亲和性结合，但不与双链DNA 结合。它还参与体内DNA 复制及重组。

原厂资料：

E. coli Single-Stranded DNA Binding Protein (SSB) consists of four identical 18.9kDa subunits. It binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA. It is involved in DNA replication and in recombination in vivo.

Applications

• Studying DNA replication and recombination.
• DNA sequencing using the primer walking strategy with contiguous oligonucleotides.

References

1. Sancar, A. et al. (1981) Proc. Natl. Acad. Sci. USA 78, 4274–8.
2. Chase, J.W. and Williams, K.R. (1986) Ann. Rev. Biochem. 55, 103–36.
3. Krauss, G. et al. (1981) Biochemistry 20, 5346–52.
4. Weiner, J.H. et al. (1975) J. Biol. Chem. 250, 1972–80.