T4 DNA Ligase

产品编号：M1801 品牌：promega 原厂货号：M1801
产品分类：克隆 > 限制性内切酶和连接酶 > 连接酶
应用分类：克隆及合成生物学 > 限制性酶切和连接 > 连接酶

包装：

100u

运保温度:

–20°C

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其它组分：

T4 DNA Ligase 10X Buffer【子货号：C126B，包装：1 x 200μl，，运保温度： –20°C】

T4 DNA Ligase【子货号：M180A，包装：1 x 100u，，运保温度： –20°C】

描述：

说明

T4 DNA Ligase(T4 DNA 连接酶) 催化粘性末端或平末端两条DNA 链的相邻核苷酸的5`- 磷酸基和3`-OH 的连接。该酶也可催化RNA 与双链DNA 或RNA 的连接，但不能使单链核酸相连。

特点

• 提供高浓度产品：Cat.# M1794 包含500 单位10–20u/μl 的T4DNA Ligase。

• 灵活：可用于5`、3` 或平末端DNA 插入片段。

• 提供10× 反应缓冲液：300mM Tris-HCl (pH 7.8,25℃ )，100mM MgCl₂，100mM DTT 和 10mM ATP。

• 蓝/白克隆验证：Promega 的蓝/ 白克隆检测为用于克隆的酶提供了高水平的质量控制。

原厂资料：

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.

The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

Features - BenefitsAvailable at High Concentration: Cat.# M1794 contains 500 units of T4 DNA Ligase at 10–20u/μl.
Flexible: Use with 5´, 3´ or blunt-ended DNA inserts.
Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.
Blue/White Cloning Qualified: Promega's blue/white cloning assay provides a higher level of quality control for enzymes used in cloning applications.
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
ApplicationsJoining double-stranded DNA molecules with cohesive or blunt ends.

注意事项：

QC Tests
Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Source
Recombinant E. coli strain.

Storage Buffer
10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Storage Conditions
Store at –20°C.

Unit Definition
0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

For product intended use please see Patents & Disclaimers tab.