收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

OneTaq Quick-Load 2X Master Mix with GC Buffer

 
包装:
运保温度: -20°C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

Description: 
OneTaq® Quick-Load® 2X Master Mix with GC Buffer is an optimized, ready-to-use blend ofTaq and Deep VentR ™ DNA Polymerases ideally suited to PCR applications from GCrich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep VentR DNA Polymerase gene from Pyrococcus species GB-D.
Applications:

  • GC-rich PCR
  • High Sensitivity PCR
  • High Throughput PCR
  • Colony PCR
  • Long PCR (up to ~6 kb genomic)

Reagents Supplied: 
OneTaq High GC Enhancer
OneTaq Quick-Load Master Mix with GC Buffer

Heat Inactivation:
No

Reaction Conditions: 
1X OneTaq Quick-Load Master Mix with GC Buffer
1X OneTaq Quick-Load Master Mix with GC Buffer:
80 mM Tris-SO4
Xylene Cyanol FF
20 mM (NH4)2SO4
0.2 mM dNTPs
2 mM MgSO4
5 % Glycerol
0.05 % Tween-20
5 % DMSO
0.06 % IGEPAL® CA-630
Tartrazine
25 units/ml OneTaq DNA Polymerase

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 
Unit Assay Conditions:
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X OneTaq Quick-Load Master Mix with GC Buffer, DNA template and primers in a total reaction volume of 50 µl.
OneTaq High GC Enhancer:
10 mM Tris-HCl
25% DMSO
25% Glycerol
pH 9.2 @ 25°C
Concentration:
2 X


相关图片

说明书

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错