OneTaq Quick-Load 2X Master Mix with GC Buffer

产品编号：M0487L 品牌：New England Biolabs 原厂货号：M0487L
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 针对高GC含量模板的PCR
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
OneTaq® Quick-Load® 2X Master Mix with GC Buffer is an optimized, ready-to-use blend ofTaq and Deep Vent_R ™ DNA Polymerases ideally suited to PCR applications from GCrich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient quick-load master mix formulation contains dNTPs, MgSO₄, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask comigrating DNA bands.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.
Applications:

GC-rich PCR
High Sensitivity PCR
High Throughput PCR
Colony PCR
Long PCR (up to ~6 kb genomic)

Reagents Supplied:
OneTaq High GC Enhancer
OneTaq Quick-Load Master Mix with GC Buffer

Heat Inactivation:
No

Reaction Conditions:
1X OneTaq Quick-Load Master Mix with GC Buffer
1X OneTaq Quick-Load Master Mix with GC Buffer:
80 mM Tris-SO₄
Xylene Cyanol FF
20 mM (NH₄)₂SO₄
0.2 mM dNTPs
2 mM MgSO₄
5 % Glycerol
0.05 % Tween-20
5 % DMSO
0.06 % IGEPAL® CA-630
Tartrazine
25 units/ml OneTaq DNA Polymerase

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
Unit Assay Conditions:
1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X OneTaq Quick-Load Master Mix with GC Buffer, DNA template and primers in a total reaction volume of 50 µl.
OneTaq High GC Enhancer:
10 mM Tris-HCl
25% DMSO
25% Glycerol
pH 9.2 @ 25°C
Concentration:
2 X