Therminator γ DNA Polymerase

• 产品编号：M0334S      品牌：New England Biolabs       原厂货号：M0334S
• 产品分类：克隆 > 聚合酶 > DNA聚合酶
• 应用分类：

描述：

Description: 
Therminator γ (gamma) DNA Polymerase is a 9°Nm™ DNA polymerase variant with an enhanced ability to incorporate gamma-phosphate labeled dNTPs.
Source:
Therminator γ DNA Polymerase is purified from a strain of E. coli that carries the 9°N (D141A / E143A / W355A / L408W / R460A / Q461S / K464E / D480V / R484W / A485L) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase fromThermococcus species 9°N-7. Developed by LI-COR® Biosciences and derived from Therminator.

Applications:

• Incorporation of modified nucleotides into DNA
• DNA sequencing using gamma-phosphate modified nucleotides

Reagents Supplied: 
Therminator γ Gamma Reaction Buffer Pack (10X) 

3´ to 5´ Exonuclease: No
5´ to 3´ Exonuclease: No
Strand Displacement: Yes

Heat Inactivation:
No

Molecular Weight: 
Theoretical: 90,000 daltons
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X Therminator γ Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13mp18.

Reaction Conditions:
1X Therminator γ Reaction Buffer, DNA template, primer, 200 µM dNTPs and 0.5–2 units of Therminator γ DNA Polymerase in a total reaction volume of 100 µl.

1X Therminator γ Reaction Buffer:
50 mM KCl
20 mM Tris-HCl
5 mM MgS0₄
0.02% IGEPAL^® CA-630
pH 9.2 @ 25°C

Concentration:
2,000 units/ml

Storage Conditions:
10 mM Tris-HCl
300 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

原厂资料：

Description: 
Therminator γ (gamma) DNA Polymerase is a 9°Nm™ DNA polymerase variant with an enhanced ability to incorporate gamma-phosphate labeled dNTPs.
Source:
Therminator γ DNA Polymerase is purified from a strain of E. coli that carries the 9°N (D141A / E143A / W355A / L408W / R460A / Q461S / K464E / D480V / R484W / A485L) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase fromThermococcus species 9°N-7. Developed by LI-COR® Biosciences and derived from Therminator.

Applications:

• Incorporation of modified nucleotides into DNA
• DNA sequencing using gamma-phosphate modified nucleotides

Reagents Supplied: 
Therminator γ Gamma Reaction Buffer Pack (10X) 

3´ to 5´ Exonuclease: No
5´ to 3´ Exonuclease: No
Strand Displacement: Yes

Heat Inactivation:
No

Molecular Weight: 
Theoretical: 90,000 daltons
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X Therminator γ Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13mp18.

Reaction Conditions:
1X Therminator γ Reaction Buffer, DNA template, primer, 200 µM dNTPs and 0.5–2 units of Therminator γ DNA Polymerase in a total reaction volume of 100 µl.

1X Therminator γ Reaction Buffer:
50 mM KCl
20 mM Tris-HCl
5 mM MgS0₄
0.02% IGEPAL^® CA-630
pH 9.2 @ 25°C

Concentration:
2,000 units/ml

Storage Conditions:
10 mM Tris-HCl
300 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C