Therminator III DNA Polymerase

• 产品编号：M0333V      品牌：New England Biolabs       原厂货号：M0333V
• 产品分类：克隆 > 聚合酶 > DNA聚合酶
• 应用分类：

描述：

Description: 
Therminator III DNA Polymerase is a 9°N DNA Polymerase variant with an enhanced ability to incorporate modified nucleotides such as 3´-amino-dNTPs, 3´-azido-dNTPs and other nucleotide analogs modified at the 3´ ribose position.

Enzyme Properties:
3´→5´ Exonuclease: No
5´→3´ Exonuclease: No
Strand Displacement: Yes

Source:
An E. coli strain that carries the 9°N (D141A / E143A / L408S / Y409A / P410V) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase fromThermococcus species 9°N-7.

Applications:

• Incorporation of nucleotide analogs with 3´-OH substitutions.
• DNA sequencing using 3´-azido-ddNTP chain terminators.
• SNP analysis with 3´-azido-ddNTP chain terminators.
• DNA labeling with 3´-amino-ddNTP.

Reagents Supplied: 
ThermoPol^® Reaction Buffer Pack (10X) 
Heat Inactivation:
No

Molecular Weight: 
Theoretical: 90,000 daltons
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed single-stranded M13mp18.

Reaction Conditions: 1X ThermoPol Reaction Buffer, DNA template, primer, 200 µM dNTPs and 0.5–2 units of Therminator III DNA Polymerase in a total reaction volume of 100 µl.

1X ThermoPol Reaction Buffer:
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton^® X-100
pH 8.8 @ 25°C

Reagents Supplied with Enzyme:
10X ThermoPol^™ Reaction Buffer.

Concentration:
2,000 units/ml

Storage Conditions:
350 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
20 mM KHPO₄
pH 6.5 @ 25°C

原厂资料：

Description: 
Therminator III DNA Polymerase is a 9°N DNA Polymerase variant with an enhanced ability to incorporate modified nucleotides such as 3´-amino-dNTPs, 3´-azido-dNTPs and other nucleotide analogs modified at the 3´ ribose position.

Enzyme Properties:
3´→5´ Exonuclease: No
5´→3´ Exonuclease: No
Strand Displacement: Yes

Source:
An E. coli strain that carries the 9°N (D141A / E143A / L408S / Y409A / P410V) DNA Polymerase gene, a genetically engineered form of the native DNA polymerase fromThermococcus species 9°N-7.

Applications:

• Incorporation of nucleotide analogs with 3´-OH substitutions.
• DNA sequencing using 3´-azido-ddNTP chain terminators.
• SNP analysis with 3´-azido-ddNTP chain terminators.
• DNA labeling with 3´-amino-ddNTP.

Reagents Supplied: 
ThermoPol^® Reaction Buffer Pack (10X) 
Heat Inactivation:
No

Molecular Weight: 
Theoretical: 90,000 daltons
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions: 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed single-stranded M13mp18.

Reaction Conditions: 1X ThermoPol Reaction Buffer, DNA template, primer, 200 µM dNTPs and 0.5–2 units of Therminator III DNA Polymerase in a total reaction volume of 100 µl.

1X ThermoPol Reaction Buffer:
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1% Triton^® X-100
pH 8.8 @ 25°C

Reagents Supplied with Enzyme:
10X ThermoPol^™ Reaction Buffer.

Concentration:
2,000 units/ml

Storage Conditions:
350 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
20 mM KHPO₄
pH 6.5 @ 25°C