Crimson Taq DNA Polymerase with (Mg-free) Buffer

• 产品编号：M0325S      品牌：New England Biolabs       原厂货号：M0325S
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
• 应用分类：

描述：

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 
Crimson Taq DNA Polymerase offers superior performance in its newly formulated PCR buffer. Crimson Taq (Mg-free) Reaction Buffer contains a density reagent, which allows direct loading of PCR products onto a gel. In addition, Crimson Taq (Mg-free) Reaction Buffer has a trace amount of a red dye, which serves as an indicator for homogenous reaction setup, a color aid in gel loading and a tracking dye which migrates at about 10 bp on a 1% TBE gel.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Applications:

• Primer Extension
• PCR
• Colony PCR
• High-throughput PCR

Advantages:

• Robust and reliable reactions
• Samples can be loaded directly onto a gel
• Ideal for high throughput applications

Reagents Supplied: 
Crimson Taq (Mg-free) Reaction Buffer Pack (5X) 
MgCl₂ (25 mM)

Heat Inactivation:
No

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X ThermoPol^® Reaction Buffer, 200 μM dNTPs including [³H]-dTTP and 200 μg/ml activated Calf Thymus DNA.

Reaction Conditions: 1X Crimson Taq (Mg-free) Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included), 1.5 mM MgCl₂ and 1.25–2.5 units of Crimson Taq DNA Polymerase in a total reaction volume of 50 µl. 

1X Crimson Taq (Mg-free) Reaction Buffer:
12.5 mM Tricine 
42.5 mM KCl
6% Dextran
Acid Red
pH 8.5 @ 25°C

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

原厂资料：