LongAmp Taq DNA Polymerase

• 产品编号：M0323S      品牌：New England Biolabs       原厂货号：M0323S
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 长片段PCR
• 应用分类：

描述：

Description: 
LongAmp® Taq DNA Polymerase is a unique blend of Taq and Deep Vent_R™ DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from human genomic DNA.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent™ DNA Polymerase gene from Pyrococcus species GB-D.

Applications:

Long Range PCR

Reagents Supplied: 
LongAmp® Taq Reaction Buffer Pack (5X) 

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 
1X LongAmp Taq Reaction Buffer, DNA template, primers, 300 µM dNTPs (not included) and 5 units of LongAmp Taq DNA Polymerase in a total reaction volume of 50 µl.

1X LongAmp Taq Reaction Buffer:
60 mM Tris-SO₄
20 mM (NH₄)₂SO₄
3 % Glycerol
0.06 % IGEPAL^® CA-630
0.05 % Tween-20
2 mM MgSO₄
pH 9.0 @ 25°C

Concentration:
2,500 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

原厂资料：

注意事项：

Store at -20°C for two years. We recommend -80°C for long term storage.