Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

• 产品编号：M0320V      品牌：New England Biolabs       原厂货号：M0320V
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
• 应用分类：

描述：

• Isolated from a recombinant source

• Supplied with 10X Reaction Buffer

• Robust and reliable reactions

• Tolerates a wide range of templates

• Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• Exceptional value in terms of cost per unit

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 
It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl₂. 10X Standard Taq(Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems. 


Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Applications:

• PCR
• Primer Extension
• DHPLC
• Microarray Analysis
• Colony PCR

Reagents Supplied: 
Standard Taq (Mg-free) Reaction Buffer Pack (10X) 
MgCl₂ (25 mM)
3´ to 5´ Exonuclease: No
5´ to 3´ Exonuclease: Yes
Strand Displacement: No
Error Rate: 285 x10^-6 bases

Heat Inactivation:
No

Molecular Weight: 
Theoretical: 94,000 daltons

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions: 1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 
1X Standard Taq (Mg-free) Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included), 1.5 mM MgCl₂ and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl.

1X Standard Taq (Mg-free) Reaction Buffer:
10 mM Tris-HCl
50 mM KCl
pH 8.3 @ 25°C

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

原厂资料：