• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• Exceptional value in terms of cost per unit
Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl2. 10X Standard Taq(Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
Applications:
PCR
Primer Extension
DHPLC
Microarray Analysis
Colony PCR
Reagents Supplied:
Standard Taq (Mg-free) Reaction Buffer Pack (10X)
MgCl2 (25 mM) 3´ to 5´ Exonuclease: No 5´ to 3´ Exonuclease: Yes Strand Displacement: No Error Rate: 285 x10-6 bases
Heat Inactivation:
No
Molecular Weight:
Theoretical: 94,000 daltons
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions: 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X Standard Taq (Mg-free) Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included), 1.5 mM MgCl2 and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl.
1X StandardTaq(Mg-free) Reaction Buffer:
10 mM Tris-HCl
50 mM KCl
pH 8.3 @ 25°C
Concentration:
5,000 units/ml
Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C