PreCR Repair Mix

• 产品编号：M0309L      品牌：New England Biolabs       原厂货号：M0309L
• 产品分类：克隆 > DNA/RNA修饰酶 > 其他酶及抑制剂
• 应用分类：

描述：

• Suitable for PCR reactions, microarrays or other DNA technologies

• Easy-to-use protocols

• Does not harm template

Description: 
The PreCR^® Repair Mix is an enzyme cocktail formulated to repair damaged template DNA prior to its use in the polymerase chain reaction (PCR), microarrays or other DNA technologies. PreCR is active on a broad range of DNA damages, including those that block PCR (e.g. apurinic/apyrimidinic sites, thymine dimers, nicks and gaps) and those that are mutagenic (e.g. deaminated cytosine and 8-oxo-guanine). In addition, it will remove a variety of moieties from the 3´end of DNA leaving a hydroxyl group. The PreCR Repair Mix will not repair all damages that inhibit/interfere with PCR. For example, it will not repair 8-oxo-7,8-dihydro-2'deoxyadenosines or fragmented DNA. In fact, the ligase present in the mix is very active at sealing nicks in DNA but does not ligate blunt ends or nicks near a mismatch effectively. This limits the possibility of chimeric gene formation as a product of ligation. The PreCR Repair Mix can be used in conjunction with any thermophilic polymerase.

Source:
Each of the recombinant proteins present in the PreCR Repair Mix has been expressed in E. coli.
Applications:

Repair of DNA prior to its use as a template in PCR or other DNA technologies.

Reagents Supplied: 
β-Nicotinamide adenine dinucleotide (NAD⁺) (100X) 
L1 Primer Mix (20 μM)
PreCR Repair Mix * (1X) 
Purified BSA (10X) 
UV damaged Lambda DNA (0.5 ng/μl)
ThermoPol^® Reaction Buffer Pack (10X)

Heat Inactivation:
No
Reaction Conditions: 
1X ThermoPol^® Reaction Buffer Pack
Incubate at 37°C.

1X ThermoPol^® Reaction Buffer Pack:
20 mM Tris-HCl
10 mM (NH₄)₂SO₄
10 mM KCl
2 mM MgSO₄
0.1 % Triton X-100
pH 8.8 @ 25°C

Storage Conditions:
20 mM Tris-HCl
100 mM NaCl
50% Glycerol
pH 7.5 @ 25°C

原厂资料：