LongAmp Taq 2X Master Mix

产品编号：M0287L 品牌：New England Biolabs 原厂货号：M0287L
产品分类：PCR/RT-PCR/qRT-PCR > PCR > 长片段PCR
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
The LongAmp^® Taq 2X Master Mix combines high quality NEB recombinant Taq DNA Polymerase with a trace amount of Deep Vent_R^® DNA Polymerase. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). This ready-to-use mix offers two fold higher fidelity than Taq DNA Polymerase alone. A wide range of PCR products can be generated, up to 30 kb from lambda DNA or from genomic DNA.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticusYT-1 and an E. coli strain that carries the Deep Vent DNA Polymerase gene fromPyrococcus species GB-D.

Applications:

Long Range PCR

Heat Inactivation:
No
Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 1X LongAmp Taq Master Mix, DNA template and primers in a total reaction volume of 25–50 µl.

1X LongAmp Taq Master Mix:
60 mM Tris-SO₄
20 mM (NH₄)₂SO₄
2 mM MgSO₄
125 units/ml LongAmp Taq DNA Polymerase
0.3 mM dNTPs
3% glycerol
0.06% IGEPAL^® CA-630
0.05% Tween-20
(pH 9.0 @ 25°C)

Concentration:
2 X