Multiplex PCR 5X Master Mix

• 产品编号：M0284S      品牌：New England Biolabs       原厂货号：M0284S
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 多重PCR
• 应用分类：

描述：

Description: 
Multiplex PCR can simultaneously detect two or more products in a single reaction. There is an increasing demand for multiplex PCR techniques in assays conducted in research laboratories and forensic/diagnostic genotyping assays (1,2). Multiplex PCR can also be used for semi-quantitative gene expression analysis using cDNA templates.

The NEB Multiplex PCR 5X Master Mix is an easy-to-use solution featuring high quality recombinant Taq DNA Polymerase. The mix is optimized for high yield and robust performance. Its performance is illustrated in a 15-plex PCR reaction using human genomic DNA (Figure 1) and an 8-plex PCR reaction using cDNA products as templates (Figure 2). The 5X formulation allows the user maximal input of customer primers, template DNAs and additional components.

Heat Inactivation:
No
Unit Definition:
One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 1X Multiplex PCR Master Mix, DNA template and primers in a total reaction volume of 25 or 50 µl.

1X Multiplex PCR Master Mix:
20 mM Tris-HCl (pH 8.9 @ 25°C)
50 mM KCl
30 mM NH₄Cl
2.5 mM MgCl₂
100 units/ml Taq DNA Polymerase
0.3 mM each dNTP
3.2% glycerol
0.08% IGEPAL^® CA-630
0.07% Tween-20

Concentration:
5 X

原厂资料：