• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• Exceptional value in terms of cost per unit
Description: Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).
Quick-Load® Taq 2X Master Mix is an optimized ready-to-use solution containingTaq DNA Polymerase, dNTPs, MgCl2, KCl, tracking dyes and stabilizers. The presence of two commonly used tracking dyes for DNA gels, Orange G and Xylene Cyanol FF, gives the master mix a green color, allowing direct loading of the PCR reaction product onto agarose gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at approximately 4 kb and Orange G migrates at approximately 50 bp. The amount of tracking dyes included does not mask co-migrating DNA bands. Quick-Load Taq 2X Master Mix is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.
Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticusYT-1.
Applications:
PCR
Primer Extension
High-Throughput PCR
Colony PCR
Reagents Supplied:
MgCl2 (25 mM) Heat Inactivation:
No Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.
Unit Assay Conditions:
1X Thermopol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.
Reaction Conditions:
1X Quick-Load Taq Master Mix, DNA template and primers in a total reaction volume of 25 or 50 µl.
1X Quick-Load Taq Master Mix:
10 mM Tris-HCl (pH 8.6 @ 25°C)
50 mM KCl
1.5 mM MgCl2
50 units/ml Taq DNA Polymerase
0.2 mM each dNTP
5% glycerol
0.08% NP-40
0.05% Tween-20
0.024% Orange G
0.0025% Xylene Cyanol FF
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