Taq DNA Polymerase with ThermoPol Buffer

• 产品编号：M0267L      品牌：New England Biolabs       原厂货号：M0267L
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
• 应用分类：

描述：

• Isolated from a recombinant source
• Supplied with 10X Reaction Buffer
• Robust and reliable reactions
• Tolerates a wide range of templates
• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• Exceptional value in terms of cost per unit

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 

It is supplied with 10X ThermoPol Reaction Buffer, which contains a nonionic detergent to increase enzyme stability during longer incubations.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticusYT-1

Applications:

• Primer extension
• PCR
• Colony PCR

Reagents Supplied: 
ThermoPol® Reaction Buffer Pack (10X) 


3´ to 5´ Exonuclease: No
5´ to 3´ Exonuclease: Yes
Strand Displacement: No
Error Rate: 285 x10-6 bases

Heat Inactivation:
No

Molecular Weight: 
Theoretical: 94,000 daltons

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions: 
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 1X ThermoPol Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included) and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl. 

1X ThermoPol Reaction Buffer:
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton X-100
pH 8.8 @ 25°C

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

原厂资料：