PRMT1 Methyltransferase

• 产品编号：M0234S      品牌：New England Biolabs       原厂货号：M0234S
• 产品分类：克隆 > DNA/RNA修饰酶 > 甲基化转移酶
• 应用分类：

描述：

Description:
PRMT1 is a major protein arginine methyltransferase (1). It specifically methylates arginine 3 (Arg 3) of H4 in vitro and in vivo. Furthermore, methylation of histone H4 at Arg 3 facilitates transcriptional activation by nuclear hormone receptor (2). Ordered cooperative functions of PRMT1, p300 and CARM1 in transcriptional activation by p53 is observed on the GADD45 gene following ectopic p53 expression and/or UV irradiation (3).

Source:
PRMT1 enzyme is expressed from rat PRMT1 cDNA using an MBP fusion tag expressed in E. coli.

Reagents Supplied:
HMT Reaction Buffer (10X)
S-adenosylmethionine (SAM) (32 mM)

Heat Inactivation:
65°C for 20 minutes

Reaction Conditions:
1X HMT Reaction Buffer
Supplemented with 160 μM S-adenosylmethionine (SAM)
Incubate at 37°C.

1X HMT Reaction Buffer:
50 mM Tris-HCl
5 mM MgCl₂
4 mM Dithiothreitol
pH 9.0 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the transfer of 1 pmol of methyl group to synthetic peptide substrate representing the first 17 amino acids of histone H4 in a total reaction volume of 25 µl in 10 minutes at 37°C.

Storage Conditions:
50 mM Tris-HCl
100 mM NaCl
5 mM MgCl₂
4 mM Dithiothreitol
50% Glycerol
pH 8.0 @ 25°C


 

原厂资料：

注意事项：

Storage Note: S-adenosylmethionine (SAM) is stored at -20°C as a 32 mM solution dissolved in 0.005 M sulfuric acid and 10% ethanol (pH 7.5). Under these conditions, SAM is stable for up to 6 months. SAM is unstable at 37°C and should be replenished in reactions incubated longer than 4 hours. Methylation can be optimized by using fresh SAM.