EcoRI Methyltransferase

• 产品编号：M0211L      品牌：New England Biolabs       原厂货号：M0211L
• 产品分类：克隆 > 限制性内切酶和连接酶 > 限制性内切酶 > E
• 应用分类：

描述：

Methylation Site:

Description:
EcoRI Methyltransferase modifies the internal adenine residue (N⁶) of the sequence GAATTC.
Source:
An E. coli. strain that carries the cloned EcoRI modification gene from Escherichia coli RY13 (R. N. Yoshimori)
Reagents Supplied:
EcoRI Methyltransferase Reaction Buffer (10X)
S-adenosylmethionine (SAM)
Molecular Weight:
Theoretical: 37,911 daltons

Reaction Conditions:
1X EcoRI Methyltransferase Reaction Buffer
Supplemented with 80 μM S-adenosylmethionine (SAM)
Incubate at 37°C.

1X EcoRI Methyltransferase Reaction Buffer:
50 mM Tris-HCl
50 mM NaCl
10 mM EDTA
pH 8.0 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by EcoRI restriction endonuclease.

Protection Assay Conditions: EcoRI Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X EcoRI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by EcoRI Methyltransferase is determined by the addition of 40 µl NEBuffer 2 and 5 units of EcoRI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on an agarose gel.

Concentration:
40,000 units/ml

Storage Conditions:
100 mM KPO₄
200 mM NaCl
10 mM 2-Mercaptoethanol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C


 

原厂资料：