The patented ON-TARGETplus modification pattern for specificity, combined with the SMARTselection algorithm for efficient, guaranteed target gene silencing, makes Thermo Scientific ON-TARGETplus siRNA the premium choice for optimal knockdown and reduced off-targets.
ON-TARGETplus SMARTpool and individual siRNAs are available as pre-designed, genome-wide reagents for human, mouse, and rat. Simply search for your gene of interest and choose from the available product formats and quantities.
Rat ON-TARGETplus siRNAs were redesigned in 2012 (RefSeq v. 54) to ensure complete genome-wide coverage and update siRNAs that no longer target all known variants. As a result, nearly 5000 new genes have been added to the collection, and over 3000 siRNAs were reversioned to more completely target known gene variants for more efficient knockdown.
Highlights
Off-targets reduced by up to 90% compared to unmodified siRNA
Guaranteed silencing by SMARTpool and 3 of 4 individual siRNAs (see Specifications tab)
Sequence information provided with siRNA purchase
A two-stranded mechanism requires a two-stranded solution
Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern:
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Seed-region analysis on siRNA designs reduces miRNA-induced off-targets
A landmark publication (see Reference 2) from the Thermo Scientific Dharmacon research group was the first to experimentally demonstrate the key role of the seed region in mediating off-targets. A subsequent 2008 paper (seeReference 3) showed the importance of seed frequency in the 3’UTR as in indicator of its likelihood to cause off-targets. These principles were subsequently applied to our siRNA designs to improve specificity:
Design filters eliminate common seed regions likely to cause miRNA-like off-targets
Seed frequency analysis for siRNA designs minimize off-target effects
Available Product Formats
SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus guaranteed to silence by 75% or better (see Specifications).
Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed to silence by 75% or better (seeSpecifications).
Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.
Individual siRNAs: Select 1, 2, or 3 individual siRNAs per gene. Minimum purchase of 4 at the 2 nmol size.
Shipping Condition
Ambient
Storage Condition
-20 C
Shelf Life
12 Months
Hazardous
No
Order Quantity Guidelines
ON-TARGETplus reagents are routinely used at 5 to 25 nM concentration. The calculations below, based on 25 nM, are for estimation purposes only and assume no loss from pipetting.
Approximate # reactions (wells) at 25 nM siRNA concentration
nmol
96-well plate
(100 µL total reaction volume)
24-well plate
(500 µL total reaction volume)
12-well plate
(1000 µL total reaction volume)
1
400
80
40
2
800
160
80
5
2000
400
200
10
4000
800
400
20
8000
1600
800
Our siRNA knockdown guarantee
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA).
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25 nM working concentration.
原厂资料：
The patented ON-TARGETplus modification pattern for specificity, combined with the SMARTselection algorithm for efficient, guaranteed target gene silencing, makes Thermo Scientific ON-TARGETplus siRNA the premium choice for optimal knockdown and reduced off-targets.
ON-TARGETplus SMARTpool and individual siRNAs are available as pre-designed, genome-wide reagents for human, mouse, and rat. Simply search for your gene of interest and choose from the available product formats and quantities.
Rat ON-TARGETplus siRNAs were redesigned in 2012 (RefSeq v. 54) to ensure complete genome-wide coverage and update siRNAs that no longer target all known variants. As a result, nearly 5000 new genes have been added to the collection, and over 3000 siRNAs were reversioned to more completely target known gene variants for more efficient knockdown.
Highlights
Off-targets reduced by up to 90% compared to unmodified siRNA
Guaranteed silencing by SMARTpool and 3 of 4 individual siRNAs (see Specifications tab)
Sequence information provided with siRNA purchase
A two-stranded mechanism requires a two-stranded solution
Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern:
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Seed-region analysis on siRNA designs reduces miRNA-induced off-targets
A landmark publication (see Reference 2) from the Thermo Scientific Dharmacon research group was the first to experimentally demonstrate the key role of the seed region in mediating off-targets. A subsequent 2008 paper (seeReference 3) showed the importance of seed frequency in the 3’UTR as in indicator of its likelihood to cause off-targets. These principles were subsequently applied to our siRNA designs to improve specificity:
Design filters eliminate common seed regions likely to cause miRNA-like off-targets
Seed frequency analysis for siRNA designs minimize off-target effects
Available Product Formats
SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus guaranteed to silence by 75% or better (see Specifications).
Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed to silence by 75% or better (seeSpecifications).
Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.
Individual siRNAs: Select 1, 2, or 3 individual siRNAs per gene. Minimum purchase of 4 at the 2 nmol size.
Shipping Condition
Ambient
Storage Condition
-20 C
Shelf Life
12 Months
Hazardous
No
Order Quantity Guidelines
ON-TARGETplus reagents are routinely used at 5 to 25 nM concentration. The calculations below, based on 25 nM, are for estimation purposes only and assume no loss from pipetting.
Approximate # reactions (wells) at 25 nM siRNA concentration
nmol
96-well plate
(100 µL total reaction volume)
24-well plate
(500 µL total reaction volume)
12-well plate
(1000 µL total reaction volume)
1
400
80
40
2
800
160
80
5
2000
400
200
10
4000
800
400
20
8000
1600
800
Our siRNA knockdown guarantee
siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA).
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25 nM working concentration.
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